Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 15, 403

In Vitro Antioxidant and Cholinesterase Inhibitory Activities of Methanolic Fruit Extract of Phyllanthus Acidus

Affiliations

In Vitro Antioxidant and Cholinesterase Inhibitory Activities of Methanolic Fruit Extract of Phyllanthus Acidus

Md Moniruzzaman et al. BMC Complement Altern Med.

Abstract

Background: Alzheimer's disease (AD) is a progressive neurodegenerative disorder clinically characterized by loss of memory and cognition. Cholinergic deficit and oxidative stress have been implicated in the pathogenesis of AD. Therefore, inhibition of acetylcholinesterase and oxidation are the two promising strategies in the development of drug for AD. Phyllanthus acidus, belonging to the family Euphorbiaceae, is a tree and has been used in traditional medicine to treat several pain, inflammatory and oxidative stress related disorders such as rheumatism, bronchitis, asthma, respiratory disorder, also important to promote intellect and enhance memory, thus supporting its possible anti-Alzheimer's properties. In this study, P. acidus was evaluated for its cholinesterase inhibitory and antioxidant activities.

Methods: In this study, we evaluated the antioxidant potential and neuroprotective activity of P. acidus by assessing total phenol content (FCR assay), total flavonoid content, total antioxidant capacity, Fe (3+) reducing power capacity, DPPH (2, 2-diphenyl-1-picrylhydrazyl) and hydroxyl radical scavenging capacity, lipid peroxidation inhibition activity & metal chelating activity. In addition acetylcholinestrase (AChE) and butyrylcholinestrase (BChE) inhibitory activities were performed using Ellman's method.

Results: Total phenolic content and total flavonoid content of the extract were 116.98 mg of gallic acid equivalent and 168.24 mg of quercetin equivalent per gm of dried extract. The methanolic extract of P. acidus (MEPA) showed considerable total antioxidant activity and reducing capacity. In DPPH scavenging assay and hydroxyl radical scavenging assay, the MEPA showed 84.33 % and 77.21 % scavenging having IC50 of 15.62 and 59.74 μg/ml respectively. In lipid peroxidation inhibition activity MEPA showed moderate inhibition of peroxidation at all concentrations with IC50 value of 471.63 μg/ml and exhibited metal chelating activity with IC50 value 308.67 μg/ml. The MEPA exhibited inhibition of rat brain acetylcholinesterase and human blood butyrylcholinesterase in a dose dependent manner and the IC50 value was found to be 1009.87 μg/ml and 449.51 μg/ml respectively.

Conclusion: These results of the present study reveal that MEPA has considerable amount of antioxidant activity as well as anti-acetylcholinesterase and anti-butyrylcholinesterase activity which suggest its effectiveness against Alzheimer's disease and other neurodegenerative disorders.

Figures

Fig. 1
Fig. 1
Total antioxidant activity of the MEPA and ascorbic acid at different concentrations
Fig. 2
Fig. 2
Reducing power capacity of the MEPA and ascorbic acid at different concentrations
Fig. 3
Fig. 3
DPPH radical scavenging activity of MEPA and BHT at different concentrations
Fig. 4
Fig. 4
IC50 (μg/ml) values of BHT and MEPA for DPPH radical scavenging activity
Fig. 5
Fig. 5
Hydroxyl radical scavenging activity of MEPA and (+)-catechin at different concentrations
Fig. 6
Fig. 6
IC50 (μg/ml) values of (+)-catechin and MEPA for Hydroxyl radical scavenging activity
Fig. 7
Fig. 7
Metal chelating activity of MEPA and BHT at different concentrations
Fig. 8
Fig. 8
IC50 (μg/ml) values of BHT and MEPA for Metal chelating activity
Fig. 9
Fig. 9
Lipid peroxidation inhibition activity of MEPA and (+)-catechin at different concentrations
Fig. 10
Fig. 10
IC50 (μg/ml) values of (+)-catechin and MEPA for Lipid peroxidation inhibition activity
Fig. 11
Fig. 11
AChE inhibitory activity of MEPA and Donepezil at different concentrations
Fig. 12
Fig. 12
IC50 (μg/ml) values of Donepezil and MEPA for AChE inhibitory activity
Fig. 13
Fig. 13
BChE inhibitory activity of MEPA and Donepezil at different concentrations
Fig. 14
Fig. 14
IC50 (μg/ml) values of Donepezil and MEPA for BChE inhibitory activity

Similar articles

See all similar articles

References

    1. Roy P, Amdekar A, Kumar A, Singh V. Preliminary study of the antioxidant properties of flowers and roots of Pyrostegiavenusta (Ker Gawl) Miers. BMC Complement Altern Med. 2011;11:69. doi: 10.1186/1472-6882-11-69. - DOI - PMC - PubMed
    1. Ayaz M, Junaid M, Ahmed M, Ullah F, Sadiq A, Ahmad S, Imran M. Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L. BMC Complement Altern Med. 2014;14:145. doi: 10.1186/1472-6882-14-145. - DOI - PMC - PubMed
    1. Jeong JM, Kang SK, Lee IH, Lee JY, Jung H, Choi CH. Antioxidant and Chemosensitizing Effects of Flavonoids with Hydroxy and or Methoxy Groups and Structure-Activity Relationship. J Pharm Pharmaceut Sci. 2007;10:537–546. - PubMed
    1. Bogdan C. Nitric oxide and the immune response. Nat Immunol. 2001;2:907. doi: 10.1038/ni1001-907. - DOI - PubMed
    1. Royer M, Stevanovic T. Study of Coryluscornuta Twig Extracts. Antioxidant, Radical Scavenging, Anti-Enzymatic Activities and Cytotoxicity. Intl J Biot Well Ind. 2012;1:67–84.

Publication types

Feedback