Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass

Sci Rep. 2015 Nov 10;5:16358. doi: 10.1038/srep16358.

Abstract

A high-density genetic map is essential for comparative genomic studies and fine mapping of QTL, and can also facilitate genome sequence assembly. Here, a high density genetic map of Asian seabass was constructed with 3321 SNPs generated by sequencing 144 individuals in a F2 family. The length of the map was 1577.67 cM with an average marker interval of 0.52 cM. A high level of genomic synteny among Asian seabass, European seabass, Nile tilapia and stickleback was detected. Using this map, one genome-wide significant and five suggestive QTL for growth traits were detected in six linkage groups (i.e. LG4, LG5, LG11, LG13, LG14 and LG15). These QTL explained 10.5-16.0% of phenotypic variance. A candidate gene, ACOX1 within the significant QTL on LG5 was identified. The gene was differentially expressed between fast- and slow-growing Asian seabass. The high-density SNP-based map provides an important tool for fine mapping QTL in molecular breeding and comparative genome analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bass / genetics*
  • Bass / growth & development*
  • Chromosome Mapping*
  • Computational Biology / methods
  • Female
  • Genetic Linkage*
  • Genomics / methods
  • High-Throughput Nucleotide Sequencing
  • Lod Score
  • Male
  • Molecular Sequence Annotation
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci*
  • Quantitative Trait, Heritable*
  • Recombination, Genetic