Immunocapture strategies in translational proteomics

Expert Rev Proteomics. 2016;13(1):83-98. doi: 10.1586/14789450.2016.1111141. Epub 2015 Nov 11.

Abstract

Aiming at clinical studies of human diseases, antibody-assisted assays have been applied to biomarker discovery and toward a streamlined translation from patient profiling to assays supporting personalized treatments. In recent years, integrated strategies to couple and combine antibodies with mass spectrometry-based proteomic efforts have emerged, allowing for novel possibilities in basic and clinical research. Described in this review are some of the field's current and emerging immunocapture approaches from an affinity proteomics perspective. Discussed are some of their advantages, pitfalls and opportunities for the next phase in clinical and translational proteomics.

Keywords: Antibodies; SRM; immunocapture; mass spectrometry; protein enrichment; sandwich assays.

Publication types

  • Review

MeSH terms

  • Animals
  • Antibodies / chemistry*
  • Humans
  • Immunoprecipitation
  • Limit of Detection
  • Mass Spectrometry
  • Protein Binding
  • Proteome / isolation & purification*
  • Proteomics / methods
  • Translational Medical Research

Substances

  • Antibodies
  • Proteome