The voltage-gated proton channel Hv1 is expressed in pancreatic islet β-cells and regulates insulin secretion

Biochem Biophys Res Commun. 2015 Dec 25;468(4):746-51. doi: 10.1016/j.bbrc.2015.11.027. Epub 2015 Nov 10.


The voltage-gated proton channel Hv1 is a potent acid extruder that participates in the extrusion of the intracellular acid. Here, we showed for the first time, Hv1 is highly expressed in mouse and human pancreatic islet β-cells, as well as β-cell lines. Imaging studies demonstrated that Hv1 resides in insulin-containing granules in β-cells. Knockdown of Hv1 with RNA interference significantly reduces glucose- and K(+)-induced insulin secretion in isolated islets and INS-1 (832/13) β-cells and has an impairment on glucose- and K(+)-induced intracellular Ca(2+) homeostasis. Our data demonstrated that the expression of Hv1 in pancreatic islet β-cells regulates insulin secretion through regulating Ca(2+) homeostasis.

Keywords: Hv1; Insulin secretion; Pancreatic islets; Voltage-gated proton channel; β-cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cell Line
  • Cells, Cultured
  • Glucose / metabolism*
  • Humans
  • Insulin / metabolism*
  • Insulin Secretion
  • Insulin-Secreting Cells / physiology*
  • Ion Channel Gating / physiology*
  • Ion Channels / physiology*
  • Mice


  • HVCN1 protein, human
  • Insulin
  • Ion Channels
  • Glucose
  • Calcium