A mutant with a defect in telomere elongation leads to senescence in yeast

Cell. 1989 May 19;57(4):633-43. doi: 10.1016/0092-8674(89)90132-3.


We describe a general assay designed to detect mutants of yeast that are defective for any of several aspects of telomere function. Using this assay, we have isolated a mutant that displays a progressive decrease in telomere length as well as an increased frequency of chromosome loss. This mutation defines a new gene, designated EST1 (for ever shorter telomeres). Null alleles of EST1 are not immediately inviable; instead, they have a senescence phenotype, due to the gradual loss of sequences essential for telomere function, leading to a progressive decrease in chromosomal stability and subsequent cell death.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology*
  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Cell Survival
  • Chromosome Aberrations
  • Chromosome Disorders
  • Chromosomes / physiology*
  • Cloning, Molecular
  • DNA / analysis
  • Molecular Sequence Data
  • Mutation*
  • Phenotype
  • Saccharomyces cerevisiae / genetics*


  • DNA

Associated data

  • GENBANK/J04849