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, 25 (2), 155-7

Interleukin 6 and STAT3 Regulate p63 Isoform Expression in Keratinocytes During Regeneration

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Interleukin 6 and STAT3 Regulate p63 Isoform Expression in Keratinocytes During Regeneration

Amanda M Nelson et al. Exp Dermatol.

Abstract

No abstract available

Keywords: hair neogenesis; p63 keratinocytes; wound-induced.

Figures

Figure 1
Figure 1. Inhibition of p63 attenuates wound-induced hair follicle neogenesis in mice
A-B) Full excision skin wounding to the depth of skeletal muscle was performed in C57BL/6 mice and 7 days after wounding (WD7), 20 μg of p63 siRNA or scrambled control (Scram) siRNA (in sterile water) was injected into the healing wound. A) Mean fold change in p63 mRNA 24 hours after injection of pan-p63 or scrambled control siRNA as determined by qRT-PCR and normalized to housekeeping gene, 18S. B) The number of regenerated hair follicles within scar, as assessed by confocal scanning laser microscopy (CSLM); representative CSLM images are shown. Area of WIHN shown within red box. Original image size is 4mm2. n =3, *p < 0.07
Figure 2
Figure 2. IL-6 and STAT3 enhance WIHN with concordant modification of p63 isoform expression in vitro and in vivo
A) Representative CSLM images for control, addition of exogenous IL-6 protein (25ng/mouse) and addition of Stat3 inhibitor, cucurbitacin I (2mg/kg). Area of WIHN shown within red box. Original image size is 4mm2. Data originally published in Nelson et al, 2015a and reproduced here for clarity. B) Mean fold change in TAp63 mRNA after IL-6 (50ng/mL) +/− cucurbitacin I in NHEK for 24 hours as determined by qRT-PCR and normalized to housekeeping gene, RPLP0. (n=4, *p< 0.05) C) In vivo TAp63 protein levels after IL-6 (25ng) compared to PBS control in C57BL/6 mice measured by western blot; normalized to β-actin (n=3, *p < 0.05). D) TAp63 and ΔNp63 protein levels after cucurbitacin I (2mg/kg) compared to PBS control in C57BL/6 mice measured by western blot; normalized to β-actin (n=3-4, *p < 0.05). E) Mean fold change in KRT1 mRNA with TAp63-specific or scrambled siRNA (control) in the presence of IL-6 (50ng/mL) in NHEK as determined by qRT-PCR and normalized to housekeeping gene, RPLP0 (n=3, *p < 0.05). Graph inset: Data originally published in Nelson et al, 2015a (Fig 4E) and reproduced here for clarity.

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