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Generalized Single-Particle cryo-EM--a Historical Perspective


Generalized Single-Particle cryo-EM--a Historical Perspective

Joachim Frank. Microscopy (Oxf).


This is a brief account of the earlier history of single-particle cryo-EM of biological molecules lacking internal symmetry, which goes back to the mid-seventies. The emphasis of this review is on the mathematical concepts and computational approaches. It is written as the field experiences a turning point in the wake of the introduction of digital cameras capable of single electron counting, and near-atomic resolution can be reached even for smaller molecules.

Keywords: 3D reconstruction; image processing; molecular structure; ribosome.

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