Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers

Zhiqiang Mei; Boxu Zhou; Chunli Wei; Jingliang Cheng; Saber Imani; Hanchun Chen; Junjiang Fu

doi:10.3390/molecules201119687

Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers

Molecules. 2015 Nov 10;20(11):20219-29. doi: 10.3390/molecules201119687.

Authors

Zhiqiang Mei¹, Boxu Zhou², Chunli Wei^{3

4}, Jingliang Cheng⁵, Saber Imani^{6

7}, Hanchun Chen⁸, Junjiang Fu^{9

10

11}

Affiliations

¹ Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. meizhiqiang@scmu.edu.cn.
² Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. 20140199120007@stu.scmu.edu.cn.
³ Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. weichunli2015@scmu.edu.cn.
⁴ State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR) 999078, China. weichunli2015@scmu.edu.cn.
⁵ Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. jingliangc@scmu.edu.cn.
⁶ Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. saberimani@scmu.edu.cn.
⁷ Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran 14359-44711, Iran. saberimani@scmu.edu.cn.
⁸ Department of Biochemistry, School of Life Sciences & the State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, China. chenhanchun@csu.edu.cn.
⁹ Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. fujunjiang@lzmc.edu.cn.
¹⁰ State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR) 999078, China. fujunjiang@lzmc.edu.cn.
¹¹ Judicial Authentication Center, Sichuan Medical University, Luzhou 646000, China. fujunjiang@lzmc.edu.cn.

Abstract

The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random ampliﬁed polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides.

Keywords: G. jasminoides Ellis var. grandiflora Nakai; Gardenia jasminoides; cloning; genetic authentication; random ampliﬁed polymorphic DNA; sequence-characterized amplified region.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
China
Cloning, Molecular
DNA, Plant*
Gardenia / classification*
Gardenia / genetics*
Genetic Markers*
Molecular Sequence Data
Random Amplified Polymorphic DNA Technique*

Substances

DNA, Plant
Genetic Markers