Nano-curcumin prepared via supercritical: Improved anti-bacterial, anti-oxidant and anti-cancer efficacy

Maobin Xie; Dejun Fan; Zheng Zhao; Zhi Li; Gang Li; Yufeng Chen; Xiaowen He; Aizheng Chen; Jiashen Li; Xiaofen Lin; Min Zhi; Yi Li; Ping Lan

doi:10.1016/j.ijpharm.2015.11.016

Nano-curcumin prepared via supercritical: Improved anti-bacterial, anti-oxidant and anti-cancer efficacy

Int J Pharm. 2015 Dec 30;496(2):732-40. doi: 10.1016/j.ijpharm.2015.11.016. Epub 2015 Nov 10.

Authors

Maobin Xie¹, Dejun Fan², Zheng Zhao³, Zhi Li⁴, Gang Li⁵, Yufeng Chen², Xiaowen He², Aizheng Chen⁶, Jiashen Li¹, Xiaofen Lin¹, Min Zhi², Yi Li⁷, Ping Lan⁸

Affiliations

¹ Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, UK.
² Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
³ State Key Lab of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 43000, China.
⁴ Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
⁵ National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
⁶ College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
⁷ Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, UK. Electronic address: henry.yili@manchester.ac.uk.
⁸ Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China. Electronic address: lanping@mail.sysu.edu.cn.

PMID: 26570985
DOI: 10.1016/j.ijpharm.2015.11.016

Abstract

Curcumin (CM) possesses multiple biological activities. However, poor water solubility and low bioavailability limit its application in biomedical fields. CM nanoparticles (NPs) (230-240nm) were prepared by solution-enhanced dispersion via supercritical CO2 (SEDS) (22-22.5MPa pressure, 31-32.5°C temperature) and its biological functions were evaluated in this study. The Minimum inhibitory concentration of CM NPs against S. aureus (∼250μg/mL) was lower than CM-DMSO (∼500μg/mL). Meanwhile, CM NPs showed effective anti-oxidant ability at a concentration raging from 125 to 2000μg/mL. CM NPs showed time-dependent intracellular internalization ability, resulting in an enhanced anti-cancer effect on colorectal cancer cells (HCT116), and the mechanism could be explained by cell cycle arrest in G2/M phase associated with inducing apoptotic cells. Moreover, CM NPs exhibited reduced cytotoxicity on normal cells (NCM460) compared to CM-DMSO and 5-Fu. In conclusion, CM NPs prepared via SEDS showed potentials in biomedical applications.

Keywords: Anti-bacterial; Anti-cancer; Anti-oxidant; Curcumin; Supercritical CO(2).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemical synthesis*
Anti-Bacterial Agents / pharmacology
Antineoplastic Agents / chemical synthesis*
Antineoplastic Agents / pharmacology
Antioxidants / chemical synthesis*
Antioxidants / pharmacology
Cell Survival / drug effects
Cell Survival / physiology
Chemistry, Pharmaceutical
Curcumin / chemical synthesis*
Curcumin / pharmacology
Dose-Response Relationship, Drug
HCT116 Cells
Humans
Nanoparticles / chemistry*
Nanoparticles / metabolism

Substances

Anti-Bacterial Agents
Antineoplastic Agents
Antioxidants
Curcumin