Peptide-conjugated hyaluronic acid surface for the culture of human induced pluripotent stem cells under defined conditions

Xiaohong Zhang; Ping Zhou; Yinghui Zhao; Mengke Wang; Shicheng Wei

doi:10.1016/j.carbpol.2015.09.081

Peptide-conjugated hyaluronic acid surface for the culture of human induced pluripotent stem cells under defined conditions

Carbohydr Polym. 2016 Jan 20:136:1061-4. doi: 10.1016/j.carbpol.2015.09.081. Epub 2015 Oct 22.

Authors

Xiaohong Zhang¹, Ping Zhou², Yinghui Zhao³, Mengke Wang⁴, Shicheng Wei⁵

Affiliations

¹ Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: zhangxiaohong@pku.edu.cn.
² Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: zhp07@pku.edu.cn.
³ Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: zhaoyinghui1987@gmail.com.
⁴ Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China. Electronic address: 874912103@qq.com.
⁵ Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: sc-wei@pku.edu.cn.

PMID: 26572447
DOI: 10.1016/j.carbpol.2015.09.081

Abstract

Hyaluronic acid (HA) has been cross-linked to form hydrogel for potential applications in the self-renewal and differentiation of human pluripotent stem cells (hPSCs) for years. However, HA hydrogel with improved residence time and mechanical integrity that allows the survival of hPSCs under defined conditions is still much needed for clinical applications. In this study, HA was modified with methacrylate functional groups (MeHA) and cross-linked by photo-crosslinking method. After subsequent conjugation with adhesive peptide, these MeHA surfaces demonstrated performance in facilitating human induced pluripotent stem cells (hiPSCs) proliferation, and good pluripotency maintenance of hiPSCs under defined conditions. Moreover, MeHA films on glass-slides exhibited long residence time and mechanical stability throughout hiPSC culture. Our photo-crosslinkable MeHA possesses great value in accelerating the application of HA hydrogel in hiPSCs proliferation and differentiation with the conjugation of adhesive peptides.

Keywords: 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (PubChem CID: 15908); Defined; Human induced pluripotent stem cell; Hyaluronic acid; Methacryloyl anhydride (PubChem CID: 12974); N-hydroxysuccinimide (PubChem CID: 80170); Peptide; Sodium hyaluronate (PubChem CID: 3084049).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods
Cell Differentiation
Cell Line
Cell Proliferation
Cross-Linking Reagents / chemistry
Humans
Hyaluronic Acid / analogs & derivatives*
Hyaluronic Acid / chemistry
Hydrogels / chemistry*
Hydrogels / pharmacology
Induced Pluripotent Stem Cells / drug effects*
Methacrylates / chemistry
Vitronectin / chemistry*

Substances

Cross-Linking Reagents
Hydrogels
Methacrylates
Vitronectin
Hyaluronic Acid