Functional analysis of alcohol dehydrogenase (ADH) genes in Pichia pastoris

Mert Karaoglan; Fidan Erden Karaoglan; Mehmet Inan

doi:10.1007/s10529-015-1993-z

Functional analysis of alcohol dehydrogenase (ADH) genes in Pichia pastoris

Biotechnol Lett. 2016 Mar;38(3):463-9. doi: 10.1007/s10529-015-1993-z. Epub 2015 Nov 14.

Authors

Mert Karaoglan¹, Fidan Erden Karaoglan¹, Mehmet Inan^{2

3}

Affiliations

¹ Department of Food Engineering, Akdeniz University, Dumlupinar Bulvari Campus, 07058, Antalya, Turkey.
² Department of Food Engineering, Akdeniz University, Dumlupinar Bulvari Campus, 07058, Antalya, Turkey. minan@akdeniz.edu.tr.
³ Food Safety and Agricultural Research Center, Akdeniz University, 07058, Antalya, Turkey. minan@akdeniz.edu.tr.

PMID: 26573637
DOI: 10.1007/s10529-015-1993-z

Abstract

Objectives: To characterize the genes responsible for ethanol utilization in Pichia pastoris.

Results: ADH3 (XM_002491337) and ADH (FN392323) genes were disrupted in P. pastoris. The ADH3 mutant strain, MK115 (Δadh3), lost its ability to grow on minimal ethanol media but produced ethanol in minimal glucose medium. ADH3p was responsible for 92 % of total Adh enzyme activity in glucose media. The double knockout strain MK117 (Δadh3Δadh) also produced ethanol. The Adh activities of X33 and MK116 (Δadh) strains were not different. Thus, the ADH gene does not play a role in ethanol metabolism.

Conclusion: The PpADH3 is the only gene responsible for consumption of ethanol in P. pastoris.

Keywords: Alcohol dehydrogenase; Crabtree yeast; Ethanol metabolism; Methylotrophic yeast; Pichia pastoris; PpADH; PpADH3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Dehydrogenase / genetics
Alcohol Dehydrogenase / metabolism*
Culture Media / chemistry
Ethanol / metabolism*
Gene Knockout Techniques
Pichia / enzymology*
Pichia / genetics
Pichia / growth & development
Pichia / metabolism*

Substances

Culture Media
Ethanol
Alcohol Dehydrogenase