A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast

Nature. 1989 Jun 1;339(6223):355-9. doi: 10.1038/339355a0.


A protein sensitive to N-ethylmaleimide catalyses the fusion of transport vesicles with Golgi cisternae in a mammalian cell-free system. By cloning and sequencing its gene from Chinese hamster ovary cells and by use of in vitro assays, we show that this fusion protein is equivalent to the SEC18 gene product of the yeast Saccharomyces cerevisiae, known to be essential for vesicle-mediated transport from the endoplasmic reticulum to the Golgi apparatus. The mechanism of vesicular fusion is thus highly conserved, both between species and at different stages of transport.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Transport
  • Carrier Proteins / genetics*
  • Carrier Proteins / physiology
  • Cell Line
  • Cell Membrane / metabolism*
  • Cytosol / metabolism
  • Ethylmaleimide / pharmacology*
  • Genes*
  • Genes, Fungal
  • Golgi Apparatus / metabolism*
  • Kinetics
  • Molecular Sequence Data
  • N-Ethylmaleimide-Sensitive Proteins
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Vesicular Transport Proteins*


  • Carrier Proteins
  • Vesicular Transport Proteins
  • N-Ethylmaleimide-Sensitive Proteins
  • Ethylmaleimide