Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover

Bioresour Technol. 2016 Jan;200:744-52. doi: 10.1016/j.biortech.2015.10.078. Epub 2015 Oct 28.


The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.

Keywords: 16S rRNA gene; Illumina sequencing; Metagenomics; Microbiome; Succession.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaerobiosis
  • Archaea / metabolism
  • Bacteria / genetics
  • Bacteria / metabolism
  • Biodiversity
  • Biofuels / analysis
  • Bioreactors / microbiology*
  • Fatty Acids, Volatile / analysis
  • Hydrogen-Ion Concentration
  • Methane / biosynthesis
  • Microbiota*
  • Phylogeny
  • Principal Component Analysis
  • Refuse Disposal / instrumentation*
  • Refuse Disposal / methods*
  • Sequence Analysis, DNA
  • Waste Disposal, Fluid*
  • Waste Products*
  • Zea mays / chemistry*


  • Biofuels
  • Fatty Acids, Volatile
  • Waste Products
  • Methane