Dandelion Extracts Protect Human Skin Fibroblasts From UVB Damage and Cellular Senescence

Oxid Med Cell Longev. 2015;2015:619560. doi: 10.1155/2015/619560. Epub 2015 Oct 20.

Abstract

Ultraviolet (UV) irradiation causes damage in skin by generating excessive reactive oxygen species (ROS) and induction of matrix metalloproteinases (MMPs), leading to skin photoageing. Dandelion extracts have long been used for traditional Chinese medicine and native American medicine to treat cancers, hepatitis, and digestive diseases; however, less is known on the effects of dandelion extracts in skin photoageing. Here we found that dandelion leaf and flower extracts significantly protect UVB irradiation-inhibited cell viability when added before UVB irradiation or promptly after irradiation. Dandelion leaf and flower extracts inhibited UVB irradiation-stimulated MMP activity and ROS generation. Dandelion root extracts showed less action on protecting HDFs from UVB irradiation-induced MMP activity, ROS generation, and cell death. Furthermore, dandelion leaf and flower but not root extracts stimulated glutathione generation and glutathione reductase mRNA expression in the presence or absence of UVB irradiation. We also found that dandelion leaf and flower extracts help absorb UVB irradiation. In addition, dandelion extracts significantly protected HDFs from H2O2-induced cellular senescence. In conclusion, dandelion extracts especially leaf and flower extracts are potent protective agents against UVB damage and H2O2-induced cellular senescence in HDFs by suppressing ROS generation and MMP activities and helping UVB absorption.

MeSH terms

  • Cell Line
  • Cell Survival / drug effects
  • Cell Survival / radiation effects
  • Cellular Senescence / drug effects*
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / radiation effects
  • Flowers / chemistry
  • Flowers / metabolism
  • Glutathione / metabolism
  • Glutathione Reductase / genetics
  • Glutathione Reductase / metabolism
  • Humans
  • Matrix Metalloproteinases / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Plant Leaves / metabolism
  • Protective Agents / pharmacology*
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism
  • Skin / cytology
  • Taraxacum / chemistry*
  • Taraxacum / metabolism
  • Ultraviolet Rays*

Substances

  • Plant Extracts
  • Protective Agents
  • RNA, Messenger
  • Reactive Oxygen Species
  • Glutathione Reductase
  • Matrix Metalloproteinases
  • Glutathione