Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry

Elio Fantini; Giovanni Giuliano

doi:10.1007/978-1-4939-3115-6_7

Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry

Methods Mol Biol. 2016:1363:65-78. doi: 10.1007/978-1-4939-3115-6_7.

Authors

Elio Fantini¹, Giovanni Giuliano²

Affiliations

¹ Trisaia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development), 75026, Rotondella, MT, Italy. elio.fantini@enea.it.
² Casaccia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development), via Anguillarese 301, 00123, Rome, Italy. giovanni.giuliano@enea.it.

PMID: 26577782
DOI: 10.1007/978-1-4939-3115-6_7

Abstract

Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.

Keywords: Reverse genetics; Silencing; TRV; Tomato; VIGS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium tumefaciens
Gene Expression Regulation, Plant
Gene Silencing*
Gene Transfer Techniques*
Genetic Vectors / genetics*
Plant Viruses / genetics*
RNA Interference
Solanum lycopersicum / genetics*
Solanum lycopersicum / metabolism*