Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus

J Clin Microbiol. 2016 Feb;54(2):439-42. doi: 10.1128/JCM.02625-15. Epub 2015 Nov 18.

Abstract

For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452 ELISA may be a preferable method for detecting antibodies against AMDV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aleutian Mink Disease / diagnosis*
  • Aleutian Mink Disease / virology
  • Aleutian Mink Disease Virus / immunology*
  • Animals
  • Antibodies, Viral / immunology*
  • Antigens, Viral / immunology*
  • Capsid Proteins / immunology*
  • Enzyme-Linked Immunosorbent Assay* / methods
  • Humans
  • Recombinant Proteins

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Capsid Proteins
  • Recombinant Proteins
  • VP2 protein, Aleutian mink disease virus