Evaluating the effect of 20-hydroxyecdysone (20HE) on mechanistic target of rapamycin complex 1 (mTORC1) signaling in the skeletal muscle and liver of rats

Appl Physiol Nutr Metab. 2015 Dec;40(12):1324-8. doi: 10.1139/apnm-2015-0301. Epub 2015 Sep 17.

Abstract

Phytoecdysteroids such as 20-hydroxyecdysone (20HE) are nutritional supplements marketed as enhancers of lean body mass. In this study the impact of 20HE ingestion on protein kinase B/Akt-mechanistic target of rapamycin complex 1 signaling in the skeletal muscle and liver of male rats was found to be limited. Bioavailability of 20HE, whether consumed alone or with leucine, also remained low at all doses ingested. Additional work is necessary to clarify 20HE mechanism of action in vivo.

Keywords: 4E-BP1; PKB/Akt; leucine; mTOR; phytoecdysteroids; phytoecdystéroïdes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Biological Availability
  • Dietary Supplements*
  • Dose-Response Relationship, Drug
  • Ecdysterone / pharmacokinetics
  • Ecdysterone / pharmacology*
  • Leucine / pharmacology
  • Liver / drug effects*
  • Liver / enzymology
  • Male
  • Mechanistic Target of Rapamycin Complex 1
  • Multiprotein Complexes / metabolism*
  • Muscle, Skeletal / drug effects*
  • Muscle, Skeletal / enzymology
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism
  • Rats, Sprague-Dawley
  • Signal Transduction / drug effects
  • TOR Serine-Threonine Kinases / metabolism*

Substances

  • Multiprotein Complexes
  • Ecdysterone
  • Mechanistic Target of Rapamycin Complex 1
  • Proto-Oncogene Proteins c-akt
  • TOR Serine-Threonine Kinases
  • Leucine