Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview

Methods Mol Biol. 2016;1355:193-209. doi: 10.1007/978-1-4939-3049-4_13.


Magnetic nanocomposites are hybrid structures consisting of an iron oxide (Fe3O4/γ-Fe2O3) superparamagnetic core and a coating shell which presents affinity for a specific target molecule. Within the scope of phosphopeptide enrichment, the magnetic core is usually first functionalized with an intermediate layer of silica or carbon to improve dispersibility and increase specific area, and then with an outer layer of a phosphate-affinity material. Fe3O4-coating materials include metal oxides, rare earth metal-based compounds, immobilized-metal ions, polymers, and many others. This chapter provides a generic overview of the different materials that can be found in literature and their advantages and drawbacks.

Keywords: Affinity; Hybrid material s; Magnetic nanocomposite s; Phosphopeptide enrich ment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aluminum Oxide / chemistry
  • Animals
  • Ferrosoferric Oxide / chemistry
  • Humans
  • Magnetite Nanoparticles / chemistry*
  • Nanotechnology*
  • Peptide Mapping
  • Phosphopeptides / analysis*
  • Phosphopeptides / chemistry
  • Phosphopeptides / metabolism
  • Phosphorylation
  • Polymers / chemistry
  • Protein Processing, Post-Translational
  • Proteomics / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Titanium / chemistry
  • Zirconium / chemistry


  • Magnetite Nanoparticles
  • Phosphopeptides
  • Polymers
  • titanium dioxide
  • Zirconium
  • Titanium
  • Aluminum Oxide
  • zirconium oxide
  • Ferrosoferric Oxide