SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing

Cell Rep. 2015 Nov 24;13(8):1647-57. doi: 10.1016/j.celrep.2015.10.038. Epub 2015 Nov 12.


Alternative splicing is a major mechanism of controlling gene expression and protein diversity in higher eukaryotes. We report that the splicing factor SRSF10 functions during striated muscle development, myoblast differentiation, and glucose production both in cells and in mice. A combination of RNA-sequencing and molecular analysis allowed us to identify muscle-specific splicing events controlled by SRSF10 that are critically involved in striated muscle development. Inclusion of alternative exons 16 and 17 of Lrrfip1 is a muscle-specific event that is activated by SRSF10 and essential for myoblast differentiation. On the other hand, in mouse primary hepatocytes, PGC1α is a key target of SRSF10 that regulates glucose production by fasting. SRSF10 represses inclusion of PGC1α exon 7a and facilitates the production of functional protein. The results highlight the biological significance of SRSF10 and regulated alternative splicing in vivo.

Keywords: alternative splicing; glucose production; myoblast differentiation; splicing factor SRSF10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics*
  • Animals
  • Cell Cycle Proteins / metabolism*
  • Cell Differentiation / genetics*
  • Cells, Cultured
  • Exons / genetics
  • Glucose / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Muscle Development / genetics
  • Myoblasts / metabolism*
  • RNA / genetics
  • RNA Splicing / genetics
  • RNA-Binding Proteins / metabolism*
  • Repressor Proteins / metabolism*
  • Sequence Analysis, RNA / methods
  • Serine-Arginine Splicing Factors


  • Cell Cycle Proteins
  • RNA-Binding Proteins
  • Repressor Proteins
  • SRSF10 protein, mouse
  • Serine-Arginine Splicing Factors
  • RNA
  • Glucose

Associated data

  • GEO/GSE66965