Microbispora camponoti sp. nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr

Antonie Van Leeuwenhoek. 2016 Feb;109(2):215-23. doi: 10.1007/s10482-015-0625-x. Epub 2015 Nov 20.


A novel actinomycete, designated strain 2C-HV3(T), was isolated from the cuticle of Camponotus japonicus Mayr collected from Harbin, Heilongjiang province, north China and characterised using a polyphasic approach. The 16S rRNA gene sequence of strain 2C-HV3(T) showed that it has high sequence similarities with Microbispora bryophytorum NEAU-TX2-2(T) (99.9 %), Microbispora amethystogenes JCM 3021(T) (98.9 %) and Microbispora rosea subsp. rosea JCM 3006(T) (98.6 %). Phylogenetic analysis based on 16S rRNA and gyrB gene sequences demonstrated that strain 2C-HV3(T) clusters with M. bryophytorum NEAU-TX2-2(T) using two tree-making algorithms. Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain 2C-HV3(T) to the genus Microbispora. Longitudinal paired spores were observed to be born on short sporophores branching from the aerial hyphae. The cell wall was found to contain meso-diaminopimelic acid as the diagnostic diamino acid; madurose was found in the whole cell hydrolysate. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, ninhydrin-positive glycophospholipids, an unidentified phospholipid and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H2) and MK-9(H4). The major fatty acids were identified as 10-methyl C17:0 and iso-C16:0. However, a combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain 2C-HV3(T) can be distinguished from its closely related relatives. Consequently, it is proposed that strain 2C-HV3(T) represents a new species of the genus Microbispora, for which the name Microbispora camponoti sp. nov. is proposed. The type strain is 2C-HV3(T) (=CGMCC 4.7281(T) = DSM 100527(T)).

Keywords: 16S rRNA gene; Camponotus japonicus Mayr; Microbispora camponoti sp. nov.; Polyphasic taxonomy; gyrB gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Actinomycetales / metabolism
  • Animal Structures / microbiology*
  • Animals
  • Ants / microbiology*
  • China
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism
  • Molecular Sequence Data
  • Phylogeny


  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids