Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)

Biochem Biophys Res Commun. 2015 Dec 25;468(4):857-62. doi: 10.1016/j.bbrc.2015.11.044. Epub 2015 Nov 14.


The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.

Keywords: Developmental growth; Genome stability; Ku70/Ku80 heterodimer; Ku80; Rice (Oryza sativa L.); Telomere.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / genetics*
  • DNA Helicases / genetics*
  • Gene Expression Regulation, Developmental / physiology*
  • Gene Expression Regulation, Plant / physiology*
  • Genomic Instability / physiology
  • Oryza / genetics*
  • Oryza / growth & development*
  • Plants, Genetically Modified / physiology
  • Telomere Homeostasis / genetics*


  • Arabidopsis Proteins
  • DNA Helicases
  • Ku80 protein, Arabidopsis