Zscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells

Biochem Biophys Res Commun. 2015 Dec 25;468(4):826-31. doi: 10.1016/j.bbrc.2015.11.039. Epub 2015 Nov 22.

Abstract

Zinc finger and SCAN domain-containing 10 (Zscan10, also known as Zfp206) encodes a transcription factor that has been reported to be involved in the maintenance of pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Zscan10 using the Cre-loxP system and analyzed its function. We succeeded in establishing Zscan10-null ES cells and confirmed their pluripotency by the generation of chimeric embryos. Our results clearly indicate that Zscan10 is dispensable for the ability of self-renewal and differentiation in ES cells.

Keywords: Embryonic stem cells; Pluripotency; Zscan10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / physiology
  • Cell Proliferation / physiology
  • Cells, Cultured
  • Mice
  • Mouse Embryonic Stem Cells / cytology*
  • Mouse Embryonic Stem Cells / physiology*
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / physiology*
  • Transcription Factors / metabolism*

Substances

  • Transcription Factors
  • ZFP206 protein, mouse