NF-κB-inducing kinase is essential for B-cell maintenance in mice

Eur J Immunol. 2016 Mar;46(3):732-41. doi: 10.1002/eji.201546081. Epub 2015 Dec 9.

Abstract

NF-κB-inducing kinase (NIK) is a key mediator of the noncanonical NF-κB signaling pathway, which is critical for normal B-cell development and function. It is well established that the complete deletion of NIK in mice results in defective B cells and impaired secondary lymphoid organogenesis. To address the role of NIK deficiency specifically in B cells, we generated a new mouse strain for the conditional deletion of this kinase. Deletion of NIK during B-cell development results in a drastic reduction of mature B cells from the transitional 2 stage on, while B-1 B cells are less affected. Moreover, deletion of NIK in the germinal centers decreases the numbers of germinal center B cells and impairs the ability of NIK-deficient B cells to develop into class-switched cells in vivo. This new mouse strain will be helpful for studying the role of NIK in different cell types of the body.

Keywords: B cell ⋅ B-1 B cells ⋅ Class switch(ing) ⋅ NF-κB-inducing kinase (NIK).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / enzymology
  • B-Lymphocytes / immunology*
  • Germinal Center / cytology
  • Germinal Center / immunology
  • Immunoglobulin Class Switching
  • Lymphocyte Activation
  • Mice
  • Protein-Serine-Threonine Kinases / deficiency
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism*
  • Signal Transduction

Substances

  • Protein-Serine-Threonine Kinases
  • NF-kappa B kinase