High frequency of Ki-ras codon 12 mutations in pancreatic adenocarcinomas

Int J Cancer. 1989 Jun 15;43(6):1037-41. doi: 10.1002/ijc.2910430614.


The frequency of Ki-ras gene mutations was studied in 100 paraffin-embedded sections obtained from 63 pancreatic adenocarcinomas by in vitro amplification of target sequences via polymerase chain reaction (PCR) and selective oligonucleotide hybridization. Forty-seven (75%) of the tumors contained a Ki-ras mutation at codon 12. No predominant amino acid substitution or nucleotide transition at this codon was observed. Two carcinomas exhibited 2 distinct Ki-ras mutations. No particular correlation could be established between the incidence of Ki-ras mutation and clinical parameters (sex, age, survival), tumor grade or tumor stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Adult
  • Aged
  • Aged, 80 and over
  • Codon / genetics*
  • DNA, Neoplasm / genetics
  • Female
  • Genes, ras*
  • Humans
  • Male
  • Middle Aged
  • Mutation*
  • Nucleic Acid Amplification Techniques
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • Pancreatic Neoplasms / genetics*
  • RNA, Messenger / genetics*


  • Codon
  • DNA, Neoplasm
  • Oligonucleotide Probes
  • RNA, Messenger