Essential oil of Algerian Eucalyptus citriodora: Chemical composition, antifungal activity

J Mycol Med. 2015 Dec;25(4):e128-33. doi: 10.1016/j.mycmed.2015.10.009. Epub 2015 Dec 1.


Objective: Essential oil of Eucalyptus citriodora is a natural product which has been attributed for various medicinal uses. In the present investigation, E. citriodora essential oil was used to evaluate its antifungal effect against medically important dermatophytes.

Material and methods: Essential oil from the Algerian E. citriodora leaves was analyzed by GC and GC/MS. The antifungal effect of E. citriodora essential oil was evaluated against four dermatophytes: Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum using disc diffusion method, disc volatilization method, and agar dilution method.

Results: The chemical composition of the oil revealed the presence of 22 compounds accounting for 95.27% of the oil. The dominant compounds were citronellal (69.77%), citronellol (10.63%) and isopulegol (4.66%). The disc diffusion method, MIC and MFC determination, indicated that E. citriodora essential oil had a higher antifungal potential against the tested strains with inhibition zone diameter which varied from (12 to 90mm) and MIC and MFC values ranged from (0.6 to 5μL/mL and 1.25 to 5μL/mL) respectively. The M. gypseum was the most resistant to the oil.

Conclusion: The results of the present study indicated that E. citriodora essential oil may be used as a new antifungal agent recommended by the pharmaceutical industries.

Keywords: Activité antifongique; Antifungal activity; Composition chimique; Dermatophytes; Essential oil; Eucalyptus Citriodora; Huile essentielle.

MeSH terms

  • Acyclic Monoterpenes
  • Aldehydes / chemistry
  • Aldehydes / pharmacology
  • Algeria
  • Antifungal Agents / chemistry*
  • Antifungal Agents / pharmacology*
  • Arthrodermataceae / drug effects
  • Arthrodermataceae / growth & development
  • Cyclohexane Monoterpenes
  • Eucalyptus / chemistry*
  • Gas Chromatography-Mass Spectrometry
  • Microbial Sensitivity Tests
  • Monoterpenes / chemistry
  • Monoterpenes / pharmacology
  • Oils, Volatile / chemistry*
  • Oils, Volatile / pharmacology*
  • Plant Leaves / chemistry
  • Plant Oils / chemistry
  • Plant Oils / pharmacology
  • Terpenes / chemistry
  • Terpenes / pharmacology


  • Acyclic Monoterpenes
  • Aldehydes
  • Antifungal Agents
  • Cyclohexane Monoterpenes
  • Monoterpenes
  • Oils, Volatile
  • Plant Oils
  • Terpenes
  • isopulegol
  • citronellol
  • citronellal