Glycine Decarboxylase Expression Increased in p53-Mutated B Cell Lymphoma Mice

Oncol Res Treat. 2015;38(11):586-9. doi: 10.1159/000441595. Epub 2015 Oct 23.

Abstract

Background: p53 gene mutations are associated with human tumors, and are among the most common genetic abnormalities. To understand the relationship between p53 mutations and glycine decarboxylase (GLDC) expression in B cell lymphoma, we established B cell lymphoma animal models to study GLDC expression in B cell lymphoma mice.

Materials and methods: Based on immunohistochemical staining results, BALB/c nude mice were divided into a p53 protein-positive group and a p53 protein-negative group. GLDC mRNA expression was determined by real-time polymerase chain reaction, and GLDC protein expression was determined by Western blot. We designed a GLDC-specific interference fragment siRNA-transfected human B cell lymphoma cell line (Raji) to establish a B cell lymphoma animal model.

Results: The results showed both GLDC mRNA and protein expression increased in the B cell lymphoma tissue of the p53 protein-positive group compared with the p53 protein-negative group. The proliferation ability of GLDC siRNA-transfected cells decreased significantly compared with the negative-control siRNA group and the blank control group (p < 0.05), which showed that the GLDC gene can promote cell proliferation in p53-mutated B cell lymphoma.

Conclusion: These studies support a direct relationship between p53 mutations and GLDC expression in B cell lymphoma. GLDC can induce dramatic changes in glycolysis and glycine/serine metabolism, leading to changes in pyrimidine metabolism and tumor development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Neoplastic
  • Glycine Dehydrogenase (Decarboxylating) / metabolism*
  • Humans
  • Lymphoma, B-Cell / enzymology*
  • Lymphoma, B-Cell / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Mutation / genetics
  • Tumor Suppressor Protein p53 / genetics*
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Tumor Suppressor Protein p53
  • Glycine Dehydrogenase (Decarboxylating)