Transformation of growth factor-dependent myeloid stem cells with retroviral vectors carrying c-myc

Oncogene. 1989 Jun;4(6):737-51.

Abstract

Myeloid progenitor cells and macrophages derived from bone marrow and spleen were efficiently transformed in vitro by infection with Moloney-based retroviral vectors carrying a human c-myc gene. Infected cells were plated in agar in the presence of combinations of the murine lymphokines CSF-1, IL-3, GM-CSF and IL-1. Between 20% and 100% of the colony-forming cells in the initial bone marrow or spleen population could be infected and gave rise to drug-resistant colonies. A large fraction of the infected cells showed continued proliferation after transfer to liquid media and we have derived over 200 growth factor-dependent cell lines. These include adherent and non-adherent CSF-1 or GM-CSF dependent macrophages and macrophage precursors and cell lines which require complex combinations of growth factors for optimal growth. Each of the cell lines displays a unique pattern of expression of surface markers specific for the myeloid lineage including the Mac-1, Mac-2, Mac-3, Ser-4 and F4/80 antigens. Surface markers not specifically associated with the myeloid lineage such as the MHC class II antigens and the Fc-receptor; and surface markers normally associated with the B-cell and T-cell lineages such as B220, L3T4 and Thy1.2 are also found on these cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / immunology
  • Blotting, Southern
  • Bone Marrow Cells
  • Cell Line
  • Cell Transformation, Neoplastic* / pathology
  • Cell Transformation, Viral*
  • Clone Cells / classification
  • Clone Cells / cytology
  • Clone Cells / immunology
  • Colony-Stimulating Factors / biosynthesis
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • Genetic Vectors
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Growth Substances / biosynthesis
  • Growth Substances / physiology
  • Hematopoietic Stem Cells / microbiology
  • Humans
  • Interleukin-3 / biosynthesis
  • Macrophages / cytology
  • Macrophages / immunology
  • Macrophages / microbiology
  • Mice
  • Proto-Oncogenes*
  • Proviruses / genetics
  • Proviruses / physiology
  • Retroviridae / genetics*
  • Retroviridae / physiology
  • Spleen / cytology

Substances

  • Antigens, Surface
  • Colony-Stimulating Factors
  • DNA, Viral
  • Growth Substances
  • Interleukin-3
  • Granulocyte-Macrophage Colony-Stimulating Factor