Comparison of the Separation of Candida Albicans Chromosome-Sized DNA by Pulsed-Field Gel Electrophoresis Techniques

Nucleic Acids Res. 1989 May 25;17(10):3783-93. doi: 10.1093/nar/17.10.3783.

Abstract

Pulsed-field gel electrophoresis techniques were used to study chromosome-sized DNA molecules of C. albicans. Chromosome-sized DNA of two strains of Candida albicans has been resolved into 8 bands by orthogonal-field-alternation gel electrophoresis (OFAGE). Six bands were observed in chromosomal preparations of C. albicans using field-inversion gel electrophoresis (FIGE). Differences in the electrophoretic mobilities of bands of the strains of C. albicans examined suggests that chromosome-length polymorphisms exist and make it difficult to correlate the banding patterns among strains. These correlations were facilitated, however, by assignment of C. albicans chromosomes by hybridization using a collection of cloned DNA probes specific for each of the 8 observed bands. Southern blotting showed that the 6 FIGE bands consisted of 4 singlets and 2 comigrating doublets, accounting for the 8 bands observed by OFAGE analysis. The agreement between OFAGE and FIGE analysis suggests that the C. albicans haploid genome contains a minimum of 8 chromosomes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Candida albicans / genetics*
  • Chromosomes / analysis*
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification*
  • Electrophoresis / methods
  • Male
  • Saccharomyces cerevisiae / genetics
  • Schizosaccharomyces / genetics

Substances

  • DNA, Fungal