Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family

Proc Natl Acad Sci U S A. 1989 Jun;86(12):4634-8. doi: 10.1073/pnas.86.12.4634.


Recently, hematopoietic stem cells were purified to homogeneity from mouse bone marrow. The protein structure of Sca-1, the cell surface antigen used in the isolation of hematopoietic stem cells, is described here. It is shown that the Sca-1 antigen is a member of the Ly-6 antigen family. The anti-Sca-1 antibody was used in immunohistochemistry experiments to define the structures in several tissues that had previously been shown to contain Ly-6 antigens. In thymus, spleen, and kidney, specific staining of parenchymal cells can be demonstrated, whereas only vasculature reacts with anti-Sca-1 in brain, heart, and liver and possibly in lung.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Ly / genetics
  • Antigens, Ly / immunology*
  • Base Sequence
  • Bone Marrow / immunology
  • DNA-Directed DNA Polymerase
  • Fluorescent Antibody Technique
  • Gene Amplification
  • Hematopoietic Stem Cells / immunology*
  • Immunoenzyme Techniques
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Spleen / immunology
  • T-Lymphocytes / immunology*
  • Transcription, Genetic


  • Antibodies, Monoclonal
  • Antigens, Ly
  • RNA, Messenger
  • DNA-Directed DNA Polymerase