Marker Detection in Electron Tomography: A Comparative Study

Microsc Microanal. 2015 Dec;21(6):1591-1601. doi: 10.1017/S1431927615015433. Epub 2015 Nov 25.


We conducted a comparative study of three widely used algorithms for the detection of fiducial markers in electron microscopy images. The algorithms were applied to four datasets from different sources. For the purpose of obtaining comparable results, we introduced figures of merit and implemented all three algorithms in a unified code base to exclude software-specific differences. The application of the algorithms revealed that none of the three algorithms is superior to the others in all cases. This leads to the conclusion that the choice of a marker detection algorithm highly depends on the properties of the dataset to be analyzed, even within the narrowed domain of electron tomography.

Keywords: electron tomography; marker detection; particle detection; tomographic reconstruction.