The new mouse genetics: altering the genome by gene targeting

Trends Genet. 1989 Mar;5(3):70-6. doi: 10.1016/0168-9525(89)90029-2.


Gene targeting (homologous recombination between DNA sequences residing in the chromosome and newly introduced DNA sequences) in pluripotent, mouse embryo-derived stem (ES) cells promises to provide the means to generate mice of any desired genotype. This review describes some of the background and current advances of gene targeting in mouse ES cells.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA, Recombinant / genetics*
  • Forecasting
  • Gene Expression Regulation
  • Genetic Engineering / methods*
  • Genetic Engineering / trends
  • Genetic Vectors
  • Mice
  • Mutation
  • Selection, Genetic
  • Stem Cells


  • DNA, Recombinant