Complex molecular genetic, karyological, and comparative morphological analyses of eelpouts of the genus Zoarces from the Sea of Okhotsk were carried out. Genetic heterogeneity of Z. elongatus and the existence of a considerably diverged form probably deserving of species status was discovered. The eelpouts caught near Shantar Islands were identified as Z. fedorovi and Z. andriashevi. The new data point to a wider distribution of these species, from Western Kamchatka to the Shantar Islands.