Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women

Sara Donzelli; Giovanni Blandino; Paola Muti

doi:10.1007/978-1-4939-3191-0_2

Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women

Methods Mol Biol. 2016:1379:13-9. doi: 10.1007/978-1-4939-3191-0_2.

Authors

Sara Donzelli¹, Giovanni Blandino², Paola Muti³

Affiliations

¹ Translational Oncogenomics Unit, Regina Elena Italian National Cancer Institute, Rome, Italy.
² Laboratory of Translational Oncogenomics, Molecular Medicine Department, Regina Elena National Cancer Institute, Rome, Italy.
³ Department of Oncology, Faculty of Health Science, McMaster University, 1280 Main Street West, Hamilton, ON, Canada. muti@mcmaster.ca.

PMID: 26608286
DOI: 10.1007/978-1-4939-3191-0_2

Abstract

MicroRNAs are key regulators of different biological processes and their deregulation is associated with the occurrence of many diseases among which cancer. Due to the higher stability of microRNAs and to the easiness in their detection both in organs than in biological fluids, many studies are turned toward potential use of this small molecules as biomarkers for the prediction and diagnosis of different types of cancer. Here we describe the experiment protocol that we have used for microRNA profiling analysis in buffy coat samples of women who developed breast cancer versus women who remained healthy during a 20 year follow-up period, with the aim to identify predictive microRNAs of breast cancer occurrence.

Keywords: Breast cancer; Buffy coat; RNA extraction; microRNA; microRNA profiling.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Blood Buffy Coat / metabolism*
Breast Neoplasms / blood*
Computational Biology / methods*
Female
Health*
Humans
Leukocytes / cytology
MicroRNAs / blood*
MicroRNAs / chemistry
MicroRNAs / genetics
MicroRNAs / isolation & purification
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis

Substances

MicroRNAs