The Methylated DNA Immunoprecipitation [MeDIP] to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development

Methods Mol Biol. 2016:1379:69-76. doi: 10.1007/978-1-4939-3191-0_6.


Epigenetic mechanisms such as DNA methylation, posttranslational modifications of histone proteins, remodeling of nucleosomes, and the expression of noncoding RNAs contribute to the regulation of gene expression for the cell fate determination and tissue development. The disruption of these epigenetic mechanisms, in conjunction with genetic alterations, is a decisive element for cancer development and progression. The cancer phenotype is characterized by global DNA hypomethylation and gene-specific hypermethylation. The methylated DNA immunoprecipitation [MeDIP] is a useful approach currently used to clarify the functional consequences of DNA methylation on cell fate determination and cancer development.

Keywords: 5-Methyl-cytosine; Cancer chemoprevention; Chromatin immunoprecipitation; CpG island; DNA methylation; Epigenetic remodeling; MeDIP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinogenesis / genetics*
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA Methylation*
  • Epigenesis, Genetic*
  • Humans
  • Immunoprecipitation / methods*
  • Neoplasms / genetics*
  • Neoplasms / pathology*
  • Polymerase Chain Reaction


  • DNA