South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anticancer Splicing Activity

Methods Mol Biol. 2016;1379:201-11. doi: 10.1007/978-1-4939-3191-0_18.

Abstract

RT-PCR is an invaluable tool for the detection and characterization of mRNA. Cancer cell lines are treated with crude plant extracts and RNA is extracted and purified with DNase prior to RT-PCR. RT-PCR first-strand cDNA synthesis is done using random primers and can be refrigerated at 4 °C. PCR from the stored cDNA is performed using transcript-specific primers and electrophoresed on a molecular grade agarose gel to separate the splice variants.

Keywords: Agarose gel electrophoresis; Anticancer splicing activity; DNase treatment; First-strand cDNA synthesis; Herbal plant extracts; RNA extraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / therapeutic use
  • Cell Line, Tumor
  • Chemoprevention
  • Electrophoresis, Agar Gel
  • Humans
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Polymerase Chain Reaction
  • RNA Splicing / drug effects*
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification

Substances

  • Antineoplastic Agents
  • Plant Extracts
  • RNA, Messenger