MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

Int J Mol Sci. 2015 Nov 24;16(11):27945-55. doi: 10.3390/ijms161126075.


Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3'UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7.

Keywords: Epithelial-Eesenchymal Transition (EMT); bone morphogenetic protein 7 (BMP7); kidney fibrosis; miR542-3p; transforming growth factor beta 1 (TGFβ1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Bone Morphogenetic Protein 7 / chemistry
  • Bone Morphogenetic Protein 7 / genetics*
  • Cell Line
  • Disease Models, Animal
  • Epithelial-Mesenchymal Transition / genetics*
  • Fibrosis
  • Gene Expression Regulation
  • Humans
  • Kidney Diseases / genetics
  • Kidney Diseases / pathology
  • Mice
  • MicroRNAs / chemistry
  • MicroRNAs / genetics*
  • RNA Interference*
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • Transforming Growth Factor beta1 / metabolism


  • Bone Morphogenetic Protein 7
  • MIRN542 microRNA, human
  • MicroRNAs
  • RNA, Messenger
  • Transforming Growth Factor beta1