Resources for the design of CRISPR gene editing experiments

Genome Biol. 2015 Nov 27;16:260. doi: 10.1186/s13059-015-0823-x.

Abstract

CRISPR-based approaches have quickly become a favored method to perturb genes to uncover their functions. Here, we review the key considerations in the design of genome editing experiments, and survey the tools and resources currently available to assist users of this technology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacterial Proteins / genetics*
  • CRISPR-Associated Protein 9
  • CRISPR-Cas Systems / genetics*
  • Endonucleases / genetics*
  • Escherichia coli / genetics
  • Gene Expression Regulation
  • Gene Targeting
  • RNA Editing / genetics*
  • RNA, Guide / genetics

Substances

  • Bacterial Proteins
  • RNA, Guide
  • CRISPR-Associated Protein 9
  • Cas9 endonuclease Streptococcus pyogenes
  • Endonucleases