Cloning and expression of a Clostridium acetobutylicum alpha-amylase gene in Escherichia coli

FEMS Microbiol Lett. 1989 May;50(1-2):135-40. doi: 10.1111/j.1574-6968.1989.tb03097.x.

Abstract

A gene library of Clostridium acetobutylicum ATCC824 was constructed in the plasmid vector pEcoR251. The library was tested for the presence of starch hydrolyzing clones. One clone in which the recombinant plasmid, pVP101, conferred alpha-amylase activity to the Escherichia coli host cell, was detected. The gene is carried on a 3.45-kbp BglII restriction fragment. A detailed physical map of pVP101 is presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular*
  • Clostridium / enzymology
  • Clostridium / genetics*
  • Escherichia coli / genetics*
  • alpha-Amylases / analysis
  • alpha-Amylases / genetics*

Substances

  • alpha-Amylases