Manipulation of the ATP pool as a tool for metabolic engineering

Biochem Soc Trans. 2015 Dec;43(6):1140-5. doi: 10.1042/BST20150141.

Abstract

Cofactor engineering has been long identified as a valuable tool for metabolic engineering. Besides interventions targeting the pools of redox cofactors, many studies addressed the adenosine pools of microorganisms. In this mini-review, we discuss interventions that manipulate the availability of ATP with a special focus on ATP wasting strategies. We discuss the importance to fine-tune the ATP yield along a production pathway to balance process performance parameters like product yield and volumetric productivity.

Keywords: adenosine 5′-triphosphate (ATP) futile cycling; metabolic engineering; product yield; volumetric productivity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Energy Metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Metabolic Engineering / methods*
  • Metabolic Networks and Pathways
  • Models, Biological
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*

Substances

  • Adenosine Triphosphate