Stimulation of neuronal cells by culture supernatant of T lymphocytes triggered by anti-CD3 mAb followed by propagation in the presence of interleukin-2

Microbiol Immunol. 2016 Jan;60(1):47-55. doi: 10.1111/1348-0421.12346.


Performance status (PS) frequently improves occurs in cancer patients who have been infused with their own lymphokine-activated killer T cells (LAK-T). In the present study, a culture supernatant of LAK-T (LAK-T sup) administered to 8-week-old rats caused neurogenesis as evidenced by increased 5-ethynyl-2'-deoxyuridine staining of brain tissues. Intravenous injection of granulocyte-macrophage colony stimulating factor (GM-CSF), a major cytokine in LAK-T sup, had a similar effect. Furthermore, LAK-T sup induced Ca(++) increase in rat hippocampal brain slices that was detected in neuronal cells by emission of Fluo-8 NW at 520 nm. The same effect was observed with an rGM-CSF solution. GM-CSF may activate neuronal cells by stimulating the glial cells that surround and attach to them. If so, GM-CSF and LAK-T sup may improve the motor neurons of patients with amyotrophic lateral sclerosis. The neurogenerative effect of GM-CSF in LAK-T sup may also help improve brain function in aged adults including those with dementia such as Alzheimer's disease.

Keywords: granulocyte-macrophage colony stimulating factor; lymphokine-activated killer T cells; neurogenesis; performance status.

MeSH terms

  • Adult
  • Alzheimer Disease / therapy
  • Amyotrophic Lateral Sclerosis / therapy
  • Animals
  • Antibodies, Monoclonal / pharmacology*
  • Brain / drug effects
  • Brain / immunology
  • Brain / pathology
  • Cell Proliferation / physiology
  • Cytokines / immunology
  • Flow Cytometry
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Immunotherapy, Adoptive / methods
  • Interleukin-2 / immunology
  • Interleukin-2 / pharmacology*
  • Killer Cells, Lymphokine-Activated / cytology
  • Killer Cells, Lymphokine-Activated / drug effects
  • Killer Cells, Lymphokine-Activated / immunology*
  • Killer Cells, Lymphokine-Activated / transplantation
  • Male
  • Neurogenesis / immunology
  • Neurons / cytology
  • Neurons / drug effects
  • Neurons / immunology*
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Wistar
  • T-Lymphocytes, Cytotoxic / drug effects*
  • T-Lymphocytes, Cytotoxic / immunology
  • Up-Regulation


  • Antibodies, Monoclonal
  • Cytokines
  • Interleukin-2
  • Granulocyte-Macrophage Colony-Stimulating Factor