Lipopolysaccharide preconditioning prevents acceleration of kindling epileptogenesis induced by traumatic brain injury

J Neuroimmunol. 2015 Dec 15:289:143-51. doi: 10.1016/j.jneuroim.2015.11.003. Epub 2015 Nov 4.

Abstract

10-20% of symptomatic epilepsies are post-traumatic. We examined effect of LPS preconditioning on epileptogenesis after controlled cortical impact (CCI). LPS (0.01, 0.1 and 0.5 mg/kg) was injected i.p. to rats 5 days before induction of CCI to parieto-temporal cortex. Kindling started 24h after CCI by i.p. injection of 30 mg/kg of pentylenetetrazole every other day until manifestation of 3 consecutive generalized seizures. CCI injury accelerated the rate of kindled seizures acquisition. LPS (0.1 and 0.5 mg/kg) prevented the acceleration of kindling. LPS preconditioning significantly decreased IL-1β and TNF-α over-expression and the number of damaged neurons in the hippocampus of traumatic rats.

Keywords: Controlled cortical impact; Epileptogenesis; LPS preconditioning; PTZ kindling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Brain Injuries / complications*
  • Brain Injuries / drug therapy
  • Convulsants / toxicity
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Drug Administration Schedule
  • Epilepsy / etiology*
  • Epilepsy / prevention & control*
  • Escherichia coli
  • Hippocampus / drug effects
  • Hippocampus / pathology
  • Interleukin-1beta / metabolism
  • Kindling, Neurologic* / drug effects
  • Lipopolysaccharides / administration & dosage*
  • Male
  • Pentylenetetrazole / toxicity
  • Rats
  • Rats, Wistar
  • Temporal Lobe / drug effects
  • Temporal Lobe / pathology
  • Time Factors

Substances

  • Convulsants
  • Interleukin-1beta
  • Lipopolysaccharides
  • Pentylenetetrazole