Pituitary tumor transforming gene PTTG2 induces psoriasis by regulating vimentin and E-cadherin expression

Int J Clin Exp Pathol. 2015 Sep 1;8(9):10887-93. eCollection 2015.


Psoriasis is a common and intractable skin disease affecting the physical and mental health of patients. This study focused on the roles of pituitary tumor transforming gene 2 (PTTG2) in psoriasis. Using real-time quantitative PCR and western blot, the expression patterns of PTTG2 were compared in psoriatic epidermis cells and normal cells, from both mRNA levels and protein levels. Knockdown of PTTG2 by siRNA was conducted in HaCaT cells to investigate the changes in cell viability and migration in vitro. Expression changes of vimentin and E-cadherin were also detected in the transfected cells. Results showed PTTG2 was significantly overexpressed in the psoriatic epidermis cells (P < 0.05). The cell viability and migration were inhibited by the knockdown of PTTG2. Besides, knockdown of PTTG2 resulted in down-regulation of vimentin and up-regulation of E-cadherin, with significant differences compared to the siRNA control group (P < 0.05). This study indicated the involvement of PTTG2 in mediating epidermis cell viability and migration and in pathogenesis of psoriasis. PTTG2 might be a potential therapeutic target for psoriasis through inducing epithelial-to-mesenchymal transition (EMT) via regulating the expression of vimentin and E-cadherin.

Keywords: E-cadherin; EMT (epithelial-to-mesenchymal transition); PTTG2 (pituitary tumor transforming gene 2); Psoriasis; vimentin.

MeSH terms

  • Antigens, CD
  • Cadherins / metabolism*
  • Case-Control Studies
  • Cell Line
  • Cell Movement
  • Cell Survival
  • Epidermis / metabolism*
  • Epidermis / pathology
  • Epithelial-Mesenchymal Transition
  • Humans
  • Keratinocytes / metabolism*
  • Keratinocytes / pathology
  • Psoriasis / genetics
  • Psoriasis / metabolism*
  • Psoriasis / pathology
  • RNA Interference
  • Securin / genetics
  • Securin / metabolism*
  • Time Factors
  • Transfection
  • Vimentin / metabolism*


  • Antigens, CD
  • CDH1 protein, human
  • Cadherins
  • Securin
  • Vimentin
  • pituitary tumor-transforming protein 2, human