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, 6 (6), 478-85
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Overexpression of miR-1260b in Non-small Cell Lung Cancer Is Associated With Lymph Node Metastasis

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Overexpression of miR-1260b in Non-small Cell Lung Cancer Is Associated With Lymph Node Metastasis

Limin Xu et al. Aging Dis.

Abstract

Lymph node (LN) metastasis is often an early event in the progression of malignant tumors and it contributes to the majority of cancer mortalities. MiRNAs play key roles in tumor metastasis. This study aimed to investigate the specific miRNAs as putative indicators of metastasis early diagnosis for non-small cell lung cancer (NSCLC). In this study, five NSCLC cases with LN metastasis and four cases without metastasis (NLN) were enrolled for Agilent Human miRNA array. The interested differentially expressed miRNA was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the LN metastasis (n = 46) and NLN (n = 39) groups. The microarray results revealed that three miRNAs (miR-1260b, miR-423-3p, miR-23a-5p) were differentially expressed in LN metastasis group compared with NLN group. The expression of miR-1260b was tested by qRT-PCR and the mean relative expression fold change (2(-ΔΔCt)) in LN metastasis was significantly higher than that in the NLN group (3.942, 1.743 respectively, P = 1.179E-04). The patients with tumor-node-metastasis (TNM) stage III were identified more frequently in LN metastasis group (P = 1.772E-11) and with a higher expression level of miR-1260b (5.126, P = 1.147E-06). In addition, the LN metastasis cases were associated with a poorly differentiated degree (P = 0.007). The overexpression of miR-1260b in NSCLC with LN metastasis can be regarded as a specific signature for early progression and prognosis of NSCLC.

Keywords: lymph node metastasis; miR-1260b; non-small cell lung cancer.

Figures

Figure 1.
Figure 1.
Change of miRNAs in the LN metastasis group. (A) Volcano plot shows the fold change of miRNAs in the LN metastasis group compared to NLN group. The vertical lines correspond to 2.0-fold up and down respectively, and the horizontal line represents a P value of 0.05. The colored points in the plot represent the differentially expressed miRNAs with statistical significance. (B) Hierarchical clustering for differentially expressed miRNAs in the LN metastasis group versus NLN group using a volcano plot (fold change ≥ 0.5). Red indicates high relative expression and green indicates low relative expression.
Figure 2.
Figure 2.
The miRNA expression level of has-miR-1260b detected by qRT-PCR in the LN metastasis and NLN groups. Error bars in the figure represent Mean ± SD. LN: lymph node metastasis; NLN: lymph node free.
Figure 3.
Figure 3.
The miRNA expression level of has-miR-1260b in different TNM stages. Error bars in the figure represent Mean ± SD.I: TNM stage I; II: TNM stage II; III: TNM stage III.

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