Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species

Methods Mol Biol. 2015;1330:101-9. doi: 10.1007/978-1-4939-2848-4_10.

Abstract

For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.

Keywords: Cellular reprogramming; Conservation; Endangered species; Induced pluripotent stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cell Differentiation
  • Cellular Reprogramming*
  • Endangered Species*
  • Feeder Cells
  • Fibroblasts / cytology*
  • Fibroblasts / metabolism*
  • Genetic Vectors / genetics
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism*
  • Mice
  • Retroviridae / genetics
  • Transcription Factors / genetics
  • Transduction, Genetic
  • Transgenes

Substances

  • Transcription Factors