Structural analysis and knock-out of a Burkholderia pseudomallei homolog of the eukaryotic transcription coactivator PC4

Gene. 2016 Feb 15;577(2):140-7. doi: 10.1016/j.gene.2015.11.037. Epub 2015 Nov 25.

Abstract

Homologs of the eukaryotic transcription coactivator PC4, which also functions in DNA repair and oxidative stress, were recently identified in prokaryotes. Crystallographic analysis of BPSL1147, a putative homolog from the pathogen Burkholderia pseudomallei K96243, reveals a highly conserved core structure and suggests a nucleic acid binding mode similar to that of PC4. Knock-out and complementation experiments do not reveal distinguishing phenotypes under normal growth conditions or in the presence of H2O2, arguing against a critical role in repair or the oxidative stress response of Burkholderia. These results may reflect redundancy or point at a bacteriophage origin of Burkholderia PC4 homologs.

Keywords: Bacteriophage; DNA replication, recombination and repair; Oxidative stress; Positive cofactor 4; Transcription.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Binding Sites
  • Burkholderia pseudomallei / genetics*
  • Burkholderia pseudomallei / metabolism
  • Molecular Sequence Data
  • Mutation
  • Oxidative Stress
  • Protein Binding
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics

Substances

  • Bacterial Proteins
  • Transcription Factors