Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding

J Microbiol. 2015 Dec;53(12):856-63. doi: 10.1007/s12275-015-5239-5. Epub 2015 Dec 2.


Lactobacillus rhamnosus CCFM1107 was screened for high antioxidative activity from 55 lactobacilli. The present study attempted to explore the protective properties of L. rhamnosus CCFM1107 in alcoholic liver injury. A mouse model was induced by orally feeding alcohol when simultaneously treated with L. rhamnosus CCFM1107, the drug Hu-Gan- Pian (HGP), L. rhamnosus GG (LGG), and L. plantarum CCFM1112 for 3 months. Biochemical analysis was performed for both serum and liver homogenate. Detailed intestinal flora and histological analyses were also carried out. Our results indicated that the administration of L. rhamnosus CCFM1107 significantly inhibited the increase in the levels of serum aminotransferase and endotoxin, as well as the levels of triglyceride (TG) and cholesterol (CHO) in the serum and in the liver. Glutathione (GSH), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were elevated while the levels of malondialdehyde (MDA) were decreased. The enteric dysbiosis caused by alcohol was restored by increasing the numbers of both lactobacilli and bifidobacteria and decreasing the numbers of both enterococci and enterobacter. Histological analysis confirmed the protective effect of L. rhamnosus CCFM1107. Compared with the other lactobacilli and to the drug Hu-Gan-Pian, there is a high chance that L. rhamnosus CCFM1107 provides protective effects on alcoholic liver injury by reducing oxidative stress and restoring the intestinal flora.

Keywords: alcoholic liver injury; endotoxin; intestinal flora; oxidative stress; probiotics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Biological Therapy / methods*
  • Cholesterol / blood
  • Disease Models, Animal
  • Drugs, Chinese Herbal / therapeutic use
  • Dysbiosis / chemically induced
  • Endotoxins / blood
  • Ethanol / adverse effects*
  • Fatty Liver, Alcoholic / prevention & control
  • Fatty Liver, Alcoholic / therapy*
  • Gastrointestinal Microbiome / drug effects
  • Glutathione / blood
  • Glutathione Peroxidase / blood
  • Lacticaseibacillus rhamnosus*
  • Liver / drug effects*
  • Liver / pathology
  • Male
  • Malondialdehyde / blood
  • Mice
  • Oxidative Stress / drug effects
  • Probiotics / therapeutic use*
  • Superoxide Dismutase / blood
  • Triglycerides / blood


  • Drugs, Chinese Herbal
  • Endotoxins
  • Triglycerides
  • Ethanol
  • Malondialdehyde
  • Cholesterol
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Alanine Transaminase
  • Glutathione