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. 2015 Dec 1;13:31.
doi: 10.1186/s12953-015-0084-3. eCollection 2015.

Hepatocytes Respond Differently to Major Dietary Trans Fatty Acid Isomers, Elaidic Acid and Trans-Vaccenic Acid

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Hepatocytes Respond Differently to Major Dietary Trans Fatty Acid Isomers, Elaidic Acid and Trans-Vaccenic Acid

Toke P Krogager et al. Proteome Sci. .
Free PMC article


Background: It has been discussed if the adverse health effect associated with the ingestion of trans fatty acids correlates with the food source, as the composition of the isomers varies in different foods. We have investigated the hepatocellular responses to the predominant trans fatty acid isomers in industrially produced partially hydrogenated vegetable oils (elaidic acid) and products of ruminant origin (trans-vaccenic acid).

Results: The responses of HepG2-SF cells exposed to 100 μM fatty acids during 7 days were examined. Elaidic acid decreased the cellular proliferation rate while trans-vaccenic acid had no effect. Analysis of cellular triacylglycerol fractions showed, that both trans fatty acids were metabolized by HepG2-SF cells, although elaidic acid, to a higher degree than trans-vaccenic, accumulated in the triacylglycerol fraction. Proteome analysis revealed that the overlap of differentially regulated proteins only contained four proteins, suggesting that the two trans fatty acid isomers affect the cells in different ways. The data are available via ProteomeXchange with identifier PXD000760.

Conclusions: Our investigations revealed that the hepatocellular response to the two most abundant dietary positional C18:1 trans fatty acid isomers differ substantially. In addition, the results suggest that trans-vaccenic acid does not affect cholesterol metabolism adversely compared to elaidic acid.

Keywords: Cardiovascular disease; Lipid metabolism; Proteomics; SILAC; Trans fatty acid.


Fig. 1
Fig. 1
Viability and proliferation of HepG2-SF cells in fatty acid supplemented medium. Viability of HepG2-SF cells in medium supplemented with 100 μM OA, cisVA, CLA, transVA, EA, or no fatty acid (Control) was investigated using CyQuant proliferation assay. The measured fluorescence (y-axis) corresponds to live cell numbers. During a seven days period cisVA, trans-VA, and Control do not markedly differ in proliferation rate, whereas EA, though still viable, appear to be compromised on their proliferation. *significantly different from Control at p < 0.05
Fig. 2
Fig. 2
The fatty acid composition of HepG2-SF TG after fatty acid supplementation. HepG2-SF TG composition was analyzed by gas liquid chromatography after 7 days incubation in fatty acid supplemented medium (100 μM). Panel a and b are high and low abundant fatty acids present in the TG, respectively. Content of individual fatty acids (x-axis), analyzed as extracted FAME, are averaged over three biological replicas and displayed as % of total TG derived FAME measured (y-axis). The supplemented TFAs esterify at different levels into HepG2-SF TG and EA is found at approximately three times the level of transVA. The analysis shows that the supplemented fatty acids are taken up and metabolized by HepG2-SF cells. In addition, it demonstrates that EA is accumulating in TG when compared to the other experimental groups. *significantly different from Control at p < 0.05. Fatty acids significantly differing between experimental groups at p < 0.05 are marked with: a) different from OA, b) different from cisVA and c) different from transVA

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