Anticancer function of α-solanine in lung adenocarcinoma cells by inducing microRNA-138 expression

Tumour Biol. 2016 May;37(5):6437-46. doi: 10.1007/s13277-015-4528-2. Epub 2015 Dec 2.


Currently, lung cancer is still a main cause of malignancy-associated death worldwide. Even though various methods for prevention and treatment of lung cancer have been improved in recent decades, the 5-year survival rate has remained very low. Insights into the anticancer function of small-molecule anticancer compounds have opened our visual field about cancer therapy. α-Solanine has been well studied for its antitumor properties, but its effect in lung cancer and associated molecular mechanisms have not yet been evaluated. To explore the anticancer function of α-solanine, we performed an MTT assay, Transwell arrays, colony-forming survival assay, quantitative reverse transcription PCR (qRT-PCR), Western blotting, and dual luciferase reporter assays in A549 and H1299 cells. We found that α-solanine not only inhibited cell migration and invasion ability but also enhanced the chemosensitivity and radiosensitivity of A549 and H1299 cells. Moreover, we discovered that α-solanine could affect the expression of miR-138 and focal adhesion kinase (FAK), both of which were also found to affect the chemosensitivity and radiosensitivity of A549 and H1299 cells. In conclusion, α-solanine could affect miR-138 and FAK expression to restrict cell migration and invasion and enhance the chemosensitivity and radiosensitivity of A549 and H1299 cells. The α-solanine/miR-138/FAK cascade can probably be a potential therapy target against lung adenocarcinoma.

Keywords: Chemosensitivity; Invasion; Lung adenocarcinoma cells; MiR-138; Migration; Radiosensitivity; α-Solanine.

MeSH terms

  • 3' Untranslated Regions
  • Adenocarcinoma / genetics*
  • Adenocarcinoma / metabolism
  • Adenocarcinoma of Lung
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Base Sequence
  • Binding Sites
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Drug Resistance, Neoplasm / drug effects
  • Focal Adhesion Protein-Tyrosine Kinases / genetics
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / metabolism
  • MicroRNAs / genetics*
  • RNA Interference
  • Radiation Tolerance / drug effects
  • Solanine / chemistry
  • Solanine / pharmacology*


  • 3' Untranslated Regions
  • Antineoplastic Agents, Phytogenic
  • MIRN138 microRNA, human
  • MicroRNAs
  • alpha-solanine
  • Solanine
  • Focal Adhesion Protein-Tyrosine Kinases