Role of cytosolic and calcium independent phospholipases A(2) in insulin secretion impairment of INS-1E cells infected by S. aureus

N Caporarello; M Salmeri; M Scalia; C Motta; C Parrino; L Frittitta; M Olivieri; M A Toscano; C D Anfuso; G Lupo

doi:10.1016/j.febslet.2015.11.035

Role of cytosolic and calcium independent phospholipases A(2) in insulin secretion impairment of INS-1E cells infected by S. aureus

FEBS Lett. 2015 Dec 21;589(24 Pt B):3969-76. doi: 10.1016/j.febslet.2015.11.035. Epub 2015 Nov 26.

Authors

N Caporarello¹, M Salmeri¹, M Scalia¹, C Motta¹, C Parrino², L Frittitta², M Olivieri¹, M A Toscano¹, C D Anfuso¹, G Lupo³

Affiliations

¹ Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
² Dept. of Clinical and Experimental Medicine, School of Medicine, University of Catania, Italy.
³ Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy. Electronic address: lupogab@unict.it.

PMID: 26632509
DOI: 10.1016/j.febslet.2015.11.035

Abstract

Cytosolic PLA2 (cPLA2) and Ca(2+)-independent PLA2 (iPLA2) play a significant role in insulin β-cells secretion. Bacterial infections may be responsible of the onset of diabetes. The mechanism by which Staphylococcus aureus infection of INS-1 cells alters glucose-induced insulin secretion has been examined. After acute infection, insulin secretion and PLA2 activities significantly increased. Moreover, increased expressions of phospho-cPLA2, phospho-PKCα and phospho-ERK 1/2 were observed. Chronic infection causes a decrease in insulin release and a significant increase of iPLA2 and COX-2 protein expression. Moreover, insulin secretion in infected cells could be restored using specific siRNAs against iPLA2 isoform and specific COX-2 inhibitor.

Keywords: INS-1E cell; Insulin; Phospholipases A(2); Staphylococcus aureus; siRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line, Tumor
Cyclooxygenase 2 / chemistry
Cyclooxygenase 2 / metabolism
Cyclooxygenase 2 Inhibitors / pharmacology
Diabetes Mellitus, Type 1 / etiology
Group IV Phospholipases A2 / metabolism*
Group VI Phospholipases A2 / antagonists & inhibitors
Group VI Phospholipases A2 / genetics
Group VI Phospholipases A2 / metabolism*
Host-Pathogen Interactions* / drug effects
Insulin / metabolism*
Insulin Secretion
Insulin-Secreting Cells / drug effects
Insulin-Secreting Cells / enzymology
Insulin-Secreting Cells / metabolism*
Insulin-Secreting Cells / microbiology
Kinetics
MAP Kinase Signaling System / drug effects
Methicillin-Resistant Staphylococcus aureus / physiology*
Pancreatitis / microbiology
Pancreatitis / physiopathology
Phosphorylation / drug effects
Protein Kinase C-alpha / metabolism
Protein Processing, Post-Translational / drug effects
RNA Interference
Rats
Staphylococcal Infections / microbiology
Staphylococcal Infections / physiopathology

Substances

Cyclooxygenase 2 Inhibitors
Insulin
Cyclooxygenase 2
Ptgs2 protein, rat
Protein Kinase C-alpha
Group IV Phospholipases A2
Group VI Phospholipases A2
Pla2g4a protein, rat
Pla2g6 protein, rat