Neuroprotective Effect of Lycopene Against PTZ-induced Kindling Seizures in Mice: Possible Behavioural, Biochemical and Mitochondrial Dysfunction

Phytother Res. 2016 Feb;30(2):306-13. doi: 10.1002/ptr.5533. Epub 2015 Dec 3.


Oxidative stress and mitochondrial dysfunction are the major contributing factors in the pathophysiology of various neurological disorders. Recently, antioxidant therapies aimed at reducing oxidative stress gained a considerable attention in epilepsy treatment. Lycopene, a carotenoid antioxidant, has received scientific interest in recent years. So, the present study has been designed to evaluate the neuroprotective effect of lycopene against the pentylenetetrazol (PTZ)-induced kindling epilepsy. Laca mice received lycopene (2.5, 5 and 10 mg/kg) and sodium valproate for a period of 29 days and PTZ (40 mg/kg i.p (Intraperitoneal)) injection on alternative days. Various behavioural (kindling score), biochemical parameters (lipid peroxidation, superoxide dismutase, reduced glutathione, catalase and nitrite) and mitochondrial enzyme complex activities (I, II and IV) were assessed in the brain. Results depicted that repeated administration of a sub-convulsive dose of PTZ (40 mg/kg) significantly increased kindling score, oxidative damage and impaired mitochondrial enzyme complex activities (I, II and IV) as compared with naive animals. Lycopene (5 and 10 mg/kg) and sodium valproate (100 mg/kg) treatment for a duration of 29 days significantly attenuated kindling score, reversed oxidative damage and restored mitochondrial enzyme complex activities (I, II and IV) as compared with control. Thus, present study demonstrates the neuroprotective potential of lycopene in PTZ-induced kindling in mice.

Keywords: lycopene; mitochondria; oxidative stress; pentylenetetrazol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Behavior, Animal / drug effects
  • Brain / drug effects
  • Carotenoids / pharmacology*
  • Catalase / metabolism
  • Dose-Response Relationship, Drug
  • Epilepsy / drug therapy
  • Glutathione / metabolism
  • Kindling, Neurologic / drug effects*
  • Lipid Peroxidation / drug effects
  • Lycopene
  • Male
  • Mice
  • Mitochondria / drug effects
  • Mitochondria / enzymology
  • Multienzyme Complexes / metabolism
  • Neuroprotective Agents / pharmacology*
  • Nitrites / metabolism
  • Oxidative Stress / drug effects
  • Pentylenetetrazole / adverse effects
  • Seizures / chemically induced
  • Seizures / drug therapy*
  • Superoxide Dismutase / metabolism


  • Antioxidants
  • Multienzyme Complexes
  • Neuroprotective Agents
  • Nitrites
  • Carotenoids
  • Catalase
  • Superoxide Dismutase
  • Glutathione
  • Lycopene
  • Pentylenetetrazole